Part:BBa_K2627006
paraBAD-crRNA targeting gltA
This part is a combination of an araBAD promoter and crRNA targeting gltA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 253
Illegal XhoI site found at 365 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 88
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 70
Results
With 0.2% L-arabinose induction, the samples showed an obvious repression on growth compared with the ones without L-arabinose (figure 2.). furthermore, we investigated the regulatory effects of targeting gltA at different stages of growth by adding L-arabinose at 0h, 2.5h, 5h, 7.5h and 9h. As shown in the figure, there presented an obvious difference between different induced time, in which the strains added L-arabinose at the beginning had the strongest repression in growth while with the delay of the induction time, the effect of inhibiting growth was gradually weakened. It indicated that this system can inhibit TCA cycle through inducing crRNA targeting gltA at different time, thus it proved that our idea that dynamically regulating metabolic flux was feasible to some extent.
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